How Much Does A Seahorse Analyzer Cost?

$25,000.00. With the Agilent Certified Pre-Owned refurbishment process, you can be confident that the Certified Pre-Owned Seahorse XFp Analyzer will provide you the same performance as a new instrument.

What is Seahorse XF Analyzer?

The XF Analyzer is the first and only commercially available instrument for scientific research that simultaneously measures in real-time and in live cells the two major energy pathways of the cell: mitochondrial respiration and glycolysis.

How does Seahorse Analyser work?

The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. In mammalian cells, glycolysis and oxidative phosphorylation (OXPHOS) pathways provide the majority of cellular ATP.

What does the Seahorse machine do?

Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis.

How does Seahorse measure oxygen?

Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive several parameters of mitochondrial respiration. Initially, baseline cellular OCR is measured, from which basal respiration can be derived by subtracting non-mitochondrial respiration.

What is oxygen consumption rate?

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells.

What is extracellular flux analyzer?

Our methodological approach relies on an extracellular flux analyzer, an instrument that enables the real-time measurement of the two central pathways used by living cells to generate adenosine triphosphate: glycolysis and oxidative phosphorylation.

How do you get a seahorse?

Buy only from reputable breeders who have been known to successfully raise captive seahorses. Seahorses raised in captivity are more expensive than wild seahorses. Captive-bred seahorses are less prone to diseases and stress than their wild counterparts and will take frozen foods in addition to fresh ones.

How does Seahorse measure ATP?

The new Seahorse XF Real-Time ATP rate assay utilizes XF technology to detect mitochondrial Oxygen Consumption Rates (OCR) and glycolytic Extra-Cellular Acidification Rates (ECAR). These data are then transformed to mitoATP and glycoATP production rates using well-validated algorithms.

How do Analysers work?

A process analyzer is an instrument that continuously or intermittently measures automatically the chemical concentration of single or multiple components, or some related physical quantity, and which provides an indication and/or a control signal of the variable(s) measured.

What is ECAR and OCR?

Changes in oxygen concentration and pH are automatically calculated and reported as Oxygen Consumption Rate (OCR) and Extra Cellular Acidification Rate (ECAR).

What is ECAR in metabolism?

Measuring the extracellular acidification rate (ECAR) provides a convenient method for detection of glycolytic flux in cancer cells in response to genetic changes, or to biologically active compounds.

What is OCR in mitochondria?

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function.

What is so special about seahorse?

Due to their unique anatomical shape and lack of scales, many people may not consider seahorses ‘fish’ at first, but they are indeed! Possessing swim bladders to remain buoyant in the water and utilizing gills to breathe, these fish also thrive in a strong suit of sturdy armor-like plates.

Is seahorse intelligent?

They have good eyesight and can see you coming clear across the room. Hippocampus is one fish that can become a true pet, and I’m convinced this is because they are more intelligent than most fishes. Seahorses are real personality fish and many of them actually enjoy being handled.

What are the 7 levels of classification for a seahorse?

Seahorses. Classification: Kingdom: Animalia, Phylum: Chordata, Class: Osteichthyes, Order: Gasterosteiformes, Family: Syngnathidae, Genus: Hippocampus, and many species. Seahorses are a marine fish belonging to the genus Hippocampus of the family Syngnathidae.

What consumes the most oxygen?

The liver, brain, and heart consume the most oxygen in the body. The liver consumes 20.4%, the brain 18.4%, and the heart 11.6%. All of the skeletal muscle combined consumes 20%, the kidneys 7.2%, and the skin 4.8%. Everything else in the body uses the remaining 17.6%.

Which organ consumes the most oxygen?

The normal human brain consumes 3.5 ml of O₂ per 100 g of brain tissue per minute, a value which remains constant throughout periods of wakefulness and sleep. This relatively high rate of oxygen consumption is appreciable when compared to the metabolic rate of the entire body.

Does walking increase VO2max?

The findings indicate that walking training of moderate intensity resulted in a modest increase in VO2max and minor but consistently favorable changes in serum lipoproteins.

How is metabolic flux measured?

How to measure flux? Metabolic flux can be inferred through the use of isotopic tracers along with Mass Spectrometry (MS) or Nuclear Magnetic Resonance (NMR). Stable-isotope-assisted methods like 13C-MFA (Metabolic Flux Analysis) is a gold standard for accurate and precise flux information.

What does FCCP do in Seahorse assay?

The FCCP-stimulated OCR can then be used to calculate spare respiratory capacity, defined as the difference between maximal respiration and basal respiration. Spare respiratory capacity is a measure of the ability of the cell to respond to increased energy demand or under stress.